Generator

Part:BBa_K3058001:Design

Designed by: Fung Chi Tuen   Group: iGEM19_Hong_Kong_LFC_PC   (2019-09-10)


Hydroxyisourate hydrolase from Cyberlindnera jadinii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To avoid codon-bias, the yeast HIU hydrolase gene is codon-optimized for better protein expression in E. coli.



Source

Sequence of HIU hydrolase gene in Cyberlindnera jadinii was codon-optimized by IDT's software and synthesized by IDT.


References